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Table 1 Polymerase chain reaction protocols used for testing ticks for selected pathogens

From: Factors associated with tick bites and pathogen prevalence in ticks parasitizing humans in Georgia, USA

Pathogen

Gene Target/Reference

Primers

Annealing Temperature

Primary/Secondary

E. chaffeensis

16S rRNA/[39]

Primary: ECC (5′-AGAACGAACGCTGGCGGCAAGCC)

55 °C/55 °C

Primary: ECB (5′-CGTATTACCGCGGCTGCTGGCA)

Secondary: HE1 (5′-CAATTGCTTATAACCTTTTGGTTATAAAT)

Secondary: HE3 (5′-TATAGGTACCGTCATTATCTTCCCTAT)

E. ewingii

16S rRNA/[39, 40]

Primary: ECC (5′-AGAACGAACGCTGGCGGCAAGCC)

55 °C/48 °C

Primary: ECB (5′-CGTATTACCGCGGCTGCTGGCA-3′)

Secondary: HE3 (5′-TATAGGTACCGTCATTATCTTCCCTAT-3′)

Secondary: EE72 (5′-CAATTCCTAAATAGTCTCTGACTATT-3′)

Rickettsia spp.

17-kDa antigen/[41, 42]

Primary: 17kD1 (5′-GCTCTTGCAACTTCTATGTT-3′)

48 °C/48 °C

Primary: 17kD2 (5′-CATTGTTCGTCAGGTTGGCG-3′)

Secondary: 17 k-5 (5′-GCTTTACAAAATTCTAAAAACCATATA)

Secondary: 17 k-3 (5′-TGTCTATCAATTCACAACTTGCC)

Borrelia spp.

Flagellin (flaB)/[43]

Primary: FLALL (5′-ACATATTCAGATGCAGACAGAGGT)

55 °C/55 °C

Primary: FLARL (5′-GCAATCATAGCCATTGCAGATTGT)

Secondary: FLALS (5′-AACAGCTGAAGAGCTTGGAATG)

Secondary: FLARS (5′-CTTTGATCACTTATCATTCTAATAGC)

F. tularensis

FopA/[44]

Primary: FNA8L (5′-CGAGGAGTCTCAATGTACTAAGGTTTGCCC)

55 °C/55 °C

Primary: FNB2L (5′-CACCATTATCCTGGATATTACCAGTGTCAT)

Secondary: FNA7L (5′-CTTGAGTCTTATGTTTCGGCATGTGAATAG)

Secondary: FNB1L (5′-CCAACTAATTGGTTGTACTGTACAGCGAAG)