Pathogen | Gene Target/Reference | Primers | Annealing Temperature |
---|---|---|---|
Primary/Secondary | |||
E. chaffeensis | 16S rRNA/[39] | Primary: ECC (5′-AGAACGAACGCTGGCGGCAAGCC) | 55 °C/55 °C |
Primary: ECB (5′-CGTATTACCGCGGCTGCTGGCA) | |||
Secondary: HE1 (5′-CAATTGCTTATAACCTTTTGGTTATAAAT) | |||
Secondary: HE3 (5′-TATAGGTACCGTCATTATCTTCCCTAT) | |||
E. ewingii | Primary: ECC (5′-AGAACGAACGCTGGCGGCAAGCC) | 55 °C/48 °C | |
Primary: ECB (5′-CGTATTACCGCGGCTGCTGGCA-3′) | |||
Secondary: HE3 (5′-TATAGGTACCGTCATTATCTTCCCTAT-3′) | |||
Secondary: EE72 (5′-CAATTCCTAAATAGTCTCTGACTATT-3′) | |||
Rickettsia spp. | Primary: 17kD1 (5′-GCTCTTGCAACTTCTATGTT-3′) | 48 °C/48 °C | |
Primary: 17kD2 (5′-CATTGTTCGTCAGGTTGGCG-3′) | |||
Secondary: 17 k-5 (5′-GCTTTACAAAATTCTAAAAACCATATA) | |||
Secondary: 17 k-3 (5′-TGTCTATCAATTCACAACTTGCC) | |||
Borrelia spp. | Flagellin (flaB)/[43] | Primary: FLALL (5′-ACATATTCAGATGCAGACAGAGGT) | 55 °C/55 °C |
Primary: FLARL (5′-GCAATCATAGCCATTGCAGATTGT) | |||
Secondary: FLALS (5′-AACAGCTGAAGAGCTTGGAATG) | |||
Secondary: FLARS (5′-CTTTGATCACTTATCATTCTAATAGC) | |||
F. tularensis | FopA/[44] | Primary: FNA8L (5′-CGAGGAGTCTCAATGTACTAAGGTTTGCCC) | 55 °C/55 °C |
Primary: FNB2L (5′-CACCATTATCCTGGATATTACCAGTGTCAT) | |||
Secondary: FNA7L (5′-CTTGAGTCTTATGTTTCGGCATGTGAATAG) | |||
Secondary: FNB1L (5′-CCAACTAATTGGTTGTACTGTACAGCGAAG) |