Reference gene stability of a synanthropic fly, Chrysomya megacephala

Parasites & Vectors

Table 1 Primers of the candidate reference genes for qPCR

Gene	Accession number	Primer sequences (5' → 3')	PCR products (bp)^a	E^a (%)	R^{2 b}
Actin	KC207081	F: ACACCATCACCAGAATCCAAG	149	97.6	0.994
		R: TTAAACCCCAAGGCTAACCG
Rpl8	KM289151	F: CTCCAAATCGGCAATGTGATG	148	93.9	0.992
		R: TCTTGGTGTCAGGGTTGTG
GAPDH	KM289150	F: AGTTATCCCTGCCTTGAACG	132	97.1	0.998
		R: AAGACCTTAGCCTTGATGTCG
EF1	FR719225	F: TTCACCGCTCAAGTCATCG	122	95.0	0.998
		R: TCGACCTTCTCCTTGATTTCAG
α-TUB	KM289152	F: GAAGGTGAATTCTCTGAGGCC	144	98.0	0.974
		R: GTCTTTTGGTTTGTGGAACGAG
β-TUB	KM289153	F: CCATTTCATCCATACCCTCACC	141	92.5	0.994
		R: GCTTGAAAATGTCTGCCACC
TBP	KM387673	F: TCATCCGCAACTCCATCTTC	127	98.7	0.991
		R: TGGGCGACATAAGACTTTGTG
18S	FJ025483	F: AGCGTATTACCGGTGGAGTTCT	78	92.5	0.994
		R:CTGAAGCAGGTTTAAATAGGAGGA
Rps7	KM289154	F: CCTTTTCACGAGCCGCTTCC	76	90.4	0.999
		R: GTGCCGGTACCCGTTACTGA

ISSN: 1756-3305