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Table 1 Primer sequences and locus characteristics.

From: Genome-wide discovery and development of polymorphic microsatellites from Leishmania panamensis parasites circulating in central Panama

Locus

Primers (5´ → 3´)a

Chrb

Locc

Repeat motif

Regiond

Tae (°C)

Fragment sizes (bp)f

M75

TCCCTAATCGTCCGTGTCAC

35

2041025

AGG

NC

56

258-270

CAGTGACGAGCTGGAAATGC

M78

AACAAGGCACGGGAAATTGG

35

2220491

AAC

NC

56

333-339

CGTCTGTTGCTCCGTCTTTG

M80

CCGCCGAGAAGCCTGAAG

29

193463

AGC

C

56

237-255

GCAACAGTACGAGAAGTGGC

M81

CATCATTCAGCAGTGCGGG

29

246586

AGG

NC

56

329-347

AAAGGAAGTGATGCGTGCAC

M140

GATTGTCGCCCTCATCAACG

17

494896

AGG

NC

64

236-263

GAGAAGAGAGGGTCGAGCTC

M147

TCTCCTTGGCTTTCCTCTCC

26

483774

AGG

NC

66

192-210

ACATATTCGCGCGCCTAATG

M149

TTCTGCCTTGTTGTTGCTCC

13

534713

AGG

NC

56

288-312

CACATCGGCCTCCATAACAC

M155

ACAAGAATGCCAAGCTGTCG

13

329823

AGC

C

62

314-323

CCTCTGCTGCTTCCTGTTTC

M166

ACTCCCTGGATAGTGGCAAC

7

107131

AGC

C

62

194-206

ATGCGGTGCGTGATGTTATG

M196

ATCAGCAGCAACTCTCCCAG

12

147953

AGC

C

62

288-300

GAGATACCGGAGACAGCGG

M202

CAGGGAGAAGAAGGGATGGC

5

267314

AGG

C

56

285-318

TCCTTTGCCTCAGTCCGTAC

M207

CTTTGACGGGCTCATCAGTG

5

251336

AGC

C

56

321-333

AAGGAGCGAGAAGTAGAGGC

M233

TCTGCGGTGAGGATGCTAC

3

102343

ACC

C

56

195-300

GCTACTACCTCTGCTCACCC

M269

TGCTGTTGACTGAATGCACTG

33

279723

AGG

NC

62

327-366

TTTCCTCCACTCCCGTCTTC

M292

GACTTAACGGATGCGCTGAC

20

158471

ACC

NC

62

240-252

GGAGCACTGAAGTAAAGCGG

M329

CCAGTCTCTTCCACCTACCC

27

715534

AGGG

NC

56

228-232

GATGGAAGGGTGCACACATC

M402

GCAGCAGTCGATTGTGAAGG

5

228009

AGAGG

NC

64

130-140

CCCTTTACATGCTGCTTGGG

  1. aUpper line, forward primer; lower line, reverse primer. Forward primer had tail attached at 5´ (GCCTCCCTCGCGCC)
  2. bChromosomal position based on L. panamensis full genome assembly
  3. cChromosomal location, considering numbering of the first nucleotide of repeat 1
  4. dLocation of microsatellite in coding (C) or non-coding (NC) regions
  5. eAnnealing temperature
  6. fRange of fragment sizes found in L. panamensis strains tested so far