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Fig. 2 | Parasites & Vectors

Fig. 2

From: A potent anti-inflammatory peptide from the salivary glands of horsefly

Fig. 2

Inhibitory effects of cecropin-TY1 on LPS-stimulated NO production in mouse peritoneal macrophages. a Inhibitory effects of cecropin-TY1 on LPS-stimulated iNOS mRNA transcription in peritoneal macrophages. The transcription levels of iNOS in peritoneal macrophages after different treatment were normalized to GAPDH. The transcription level of iNOS in macrophages induced by 100 ng/mL LPS was arbitrarily defined as 100 %. Data were presented as mean ± SEM. *P <0.05, **P < 0.01, values of peptide-treated groups are significantly different from that induced by 100 ng/mL LPS alone. b Inhibitory effects of cecropin-TY1 on LPS-stimulated nitrite production in peritoneal macrophages. Peritoneal macrophages were stimulated with or without LPS (100 ng/mL), then different concentrations of cecropin-TY1 (cec-TY1, 5, 10, 20 μg/mL) were added as indicated and incubated for 6 h to detect iNOS transcription levels and 24 h to detect nitrite accumulation levels, respectively. Data were presented as mean ± SEM. *P <0.05, **P < 0.01, values of peptide-treated groups are significantly different from that induced by 100 ng/mL LPS alone. c Effects of different derivatives of cecropin-TY1 on LPS-stimulated nitrite production. Peritoneal macrophages were stimulated with 100 ng/mL LPS, then peptides (5 μM) were added as indicated and incubated for 24 h to detect nitrite accumulation levels in the culture medium. Data were presented as mean ± SEM. *P <0.05, **P < 0.01, values of derivative-treated groups are significantly different from that of cecropin-TY1-treated group

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