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Estimation of changes in the force of infection for intestinal and urogenital schistosomiasis in countries with schistosomiasis control initiative-assisted programmes
Parasites & Vectors volume 8, Article number: 558 (2015)
The last decade has seen an expansion of national schistosomiasis control programmes in Africa based on large-scale preventative chemotherapy. In many areas this has resulted in considerable reductions in infection and morbidity levels in treated individuals. In this paper, we quantify changes in the force of infection (FOI), defined here as the per (human) host parasite establishment rate, to ascertain the impact on transmission of some of these programmes under the umbrella of the Schistosomiasis Control Initiative (SCI).
A previous model for the transmission dynamics of Schistosoma mansoni was adapted here to S. haematobium. These models were fitted to longitudinal cohort (infection intensity) monitoring and evaluation data. Changes in the FOI following up to three annual rounds of praziquantel were estimated for Burkina Faso, Mali, Niger, Tanzania, Uganda, and Zambia in sub-Saharan Africa (SSA) according to country, baseline endemicity and schistosome species. Since schistosomiasis transmission is known to be highly focal, changes in the FOI at a finer geographical scale (that of sentinel site) were also estimated for S. mansoni in Uganda.
Substantial and statistically significant reductions in the FOI relative to baseline were recorded in the majority of, but not all, combinations of country, parasite species, and endemicity areas. At the finer geographical scale assessed within Uganda, marked heterogeneity in the magnitude and direction of the relative changes in FOI was observed that would not have been appreciated by a coarser-scale analysis.
Reductions in the rate at which humans acquire schistosomes have been achieved in many areas of SSA countries assisted by the SCI, while challenges in effectively reducing transmission persist in others. Understanding the underlying heterogeneity in the impact and performance of the control intervention at the level of the transmission site will become increasingly important for programmes transitioning from morbidity reduction to elimination of infection. Such analyses will require a fine-scale approach. The lack of association found between programmatic variables, such as therapeutic treatment coverage (recorded at district level) and changes in FOI (at sentinel site level) is discussed and recommendations are made.
Recent years have seen a great expansion of national schistosomiasis control programmes, mostly based on large-scale preventive chemotherapy—also known as mass drug administration (MDA)—with praziquantel, resulting in significant successes in controlling infection prevalence, intensity, and morbidity [1, 2]. What is not yet clear is the benefit of such large-scale treatment to the wider community via reductions in transmission. These reductions will be manifested via a decrease in the force of infection (FOI) which, for macroparasitic infections, is defined as the per capita rate at which a host acquires new infections . The precise understanding of the FOI can be interpreted in a number of different ways according to which stage of the parasites’ life-cycle is of interest. The number of adult schistosomes within a host can rarely be measured directly, so parasitological surveys typically rely on faecal egg counts as a proxy for parasite intensity. Therefore, routinely used diagnostic tools cannot identify newly established parasites until they reach patency and reproduce successfully.
For the purposes of this paper, the FOI is defined as the rate at which new incoming worms establish into adult parasites and reach patency (initiate detectable egg production) in the human host population, and is conceptually similar to the use of age-specific rates of reinfection after treatment to measure the force of infection used by other researchers . However, this may differ from the rate at which the host population is infected by other parasitic stages, such as by cercariae from the environment, which has been the approach used historically via the use of snail studies or cercariometry [5–7].
Since schistosomiasis transmission models began to be developed in earnest in the 1960s, significant advances were the formulation of the reproduction number (in prevalence models) to estimate rates of infection and clearance [8, 9]; inclusion of mating probabilities and transmission breakpoints (in intensity frameworks) to examine how these influence the establishment, persistence, and elimination of the infection [10–12]; incorporation of parasite latency and snail mortality [3, 12–14]; and the comparison of model outputs to field data to investigate heterogeneity in infection , among others. Although some authors have favoured the use of prevalence-based models , the use of (deterministic) immigration-death, intensity frameworks was adopted in , where such approaches describe the rate of change of the number of schistosomes per person with respect to time and host age. These were used to estimate the rate of parasite acquisition (FOI), with this rate found to be reliant on the intensity of incoming worms and the load of already established parasites. Although such models track the number of adult worms in human hosts, they either calculate the prevalence of patent infections in snails [18, 19], or assume that the schistosome population in snails and the parasite larval population (miracidia; cercariae) in the environment are at equilibrium given the disparity in the respective life-spans . The latter was the approach adopted by the authors of the deterministic model EpiSchisto®, upon which the code used in this study is based.
In a previous paper we used this approach to estimate changes in the FOI relative to that at baseline, for cohorts of individuals treated annually for three years with praziquantel against intestinal schistosomiasis in Uganda . The extensive datasets of the Schistosomiasis Control Initiative (SCI), allowed regions within Uganda to be classified according to underlying levels of average infection intensity at baseline measured in a cohort of school-aged children and adults, namely, high endemicity: ≥400 eggs per gram of faeces [epg]; moderate endemicity: 100–399 epg, and low endemicity: 1–99 epg . Substantial and statistically significant reductions in the FOI were observed following a single treatment round in areas of low intensity of infection (76 % reduction; 95 % confidence interval [95 % CI]: 59–100 %), and following two rounds in those of moderate (66 %; 95 % CI: 26–87 %) and high (63 %; 95 % CI: 37–78 %) intensity. It was concluded that the programme not only benefited those individuals receiving treatment, but also those who missed treatment, due to the ensuing decrease in environmental transmission. Demonstrating quantitatively the impact of anthelmintic interventions is crucial to ensure their sustainability, the engagement of communities and funders, and the ultimate estimation of their cost effectiveness .
Notwithstanding the usefulness of examining the impact of interventions at relatively broad epidemiological levels, these are likely to combine areas that differ in terms of their transmission patterns. In reality, schistosomiasis is a focal disease, and considerable heterogeneity can often be observed in infection in neighbouring communities, and even within communities . In this paper the earlier modelling approach is extended to calculate relative changes in the FOI according to endemicity level in six countries that received SCI support between 2003 and 2008 for both Schistosoma mansoni and S. haematobium. The heterogeneity of such results at a smaller, finer geographical scale is also explored, using S. mansoni in Uganda as an illustrative example.
Datasets and cohorts
A full description of the data collection design for the monitoring and evaluation (M&E) components of the SCI treatment programmes is given elsewhere . In brief, data were collected for the six countries that implemented national or near-national treatment for schistosomiasis (and soil-transmitted helminthiasis), soon after the inception of the SCI in 2002. These were three countries from West Africa (Burkina Faso, Mali, Niger), and three from East Africa (Tanzania, Uganda, Zambia), which provided datasets on either S. mansoni or S. haematobium or both (Table 1 and Fig. 1). The M&E approach was designed using a statistical framework  to ensure that were a reduction in infection levels occurring, such a reduction would be identifiable and measurable .
There are two types of cohorts that comprise the SCI data. Longitudinal cohorts consist of school-age children in primary education (6–12 years or 7–12 years depending on age at first enrolment) followed up every year for 1 year (Zambia) or 2–3 years (the remaining countries) (Table 1). Community cohorts focus on collecting data from adults but also include children (age range of 4 – 88 years); these are designed to capture individuals longitudinally but due to the higher drop-out rate are often of a cross-sectional design.
M&E cohorts are often assumed (either explicitly or implicitly) to be representative of the wider community. This may be true at baseline, where extra efforts are often made to attract non-enrolled school-age children. However, at follow-up, when (systematic) non-compliance may play a significant role, those children more heavily infected or too ill, engaged in work, or living in more distant locations may not be able to attend school for re-treatment. To investigate whether the longitudinal cohorts were truly representative of the study populations of interest, the baseline values of infection intensity and prevalence in those children who were followed for each year of the study were compared with those of children who were lost to follow-up  via normal distribution z-tests for large samples . The cohort retention rate was calculated as the proportion of individuals recruited into the cohort at baseline who subsequently were positively identified at each of the following treatment rounds. Differences in the cohort retention rate with respect to age were calculated using logistic regression with age as the dependent variable and whether the individual was retained in the cohort as the independent variable.
Sampling and descriptive statistics
For S. mansoni, infection intensity was estimated as the arithmetic mean of either four Kato-Katz slides taken across two consecutive days (two slides on day one and two slides on day two, in Uganda, Tanzania, and Zambia) or of two Kato-Katz slides taken on one day (Mali, Niger, Burkina Faso). Infection intensity is expressed as eggs per gram of faeces (epg) . For S. haematobium, infection intensity was estimated from either a single urine filtration sample (Uganda, Tanzania, Zambia, Mali, and Burkina Faso) or a double urine filtration from a single urine sample (Niger). Infection intensity is expressed as eggs per 10 ml urine (e/10 ml) .
The relationship between adult worm burden and egg output is poorly understood. This is in part caused by variations in egg output by day, uneven distribution of eggs in the excreta, imperfect sensitivity of diagnostic methods, and the fact that only a proportion of the eggs will exit the body [29–33]. However, attempts to define the relationship between egg output and underlying worm burden have concluded that there may be a positive and linear relationship for both S. mansoni  and S. haematobium . In contrast, analysis of the human autopsy dataset of Cheever  suggested some measure of negative density dependence in S. mansoni . In the present study, and for parsimony, a density-independent relationship was assumed between female worm burden and egg output, with each S. mansoni female producing on average of 5.26 epg , and each S. haematobium female a mean of 3.60 e/10 ml .
Arithmetic means of infection intensity were used as measures of central tendency , and their ninety five percent confidence intervals (95 % CI) were calculated using the normal approximation for large sample sizes . Point estimates of prevalence of infection and categories of infection (as described below) were also calculated and the normal approximation to the binomial distribution was used to estimate their 95 % CI given the large sample sizes available . All statistical analyses were carried out in the freely available software R .
To follow the same methodology for categorization of endemicity of French et al. , the datasets were classified into areas of low, moderate and high underlying endemicity, based on the average infection intensity for each sentinel site (school/community) at baseline, prior to large-scale implementation of SCI-supported praziquantel treatment programmes (although the possibility that there had been previous, smaller-scale ad-hoc treatment programmes cannot be totally discounted). Intestinal schistosomiasis areas were allocated into high (≥400 epg), moderate (100–399 epg) and low (1–99 epg) infection intensity areas as described above. For urogenital schistosomiasis, areas they were classified into high (≥50 e/10 ml) and low (1–49 e/10 ml) intensity areas . In both Mali and Tanzania the numbers of highly-infected S. mansoni sentinel sties was low, and so these were combined into a single high/moderate category.
Full details of the population dynamics model for S. mansonican be found in French et al. (2010)  and in the supplementary information of that paper. These authors modified an earlier framework presented by Chan et al.  known as EpiSchisto®. In turn, EpiSchisto® was based on previous work by Anderson and May [3, 17] developing the use of immigration-death models. The rate of change in mean adult S. mansoniworm burden (M) (after the conversion from egg output to adult female burden given previously of 5.26 epg per mated female worm) with respect to host age (a) and time (t) can be described by the following immigration-death equation,
where Λ(a) is the net FOI at age a, and μ M is the per worm death rate of established (mature) adult worms. In turn, Λ(a) is given by Eq. 2,
Here, λ B is the average underlying baseline FOI per person, ζ P is the relative to baseline ratio of the average FOI after each round of treatment, with subscript P indicating the number of rounds of praziquantel treatments received, and the function τ(a) describes the (dimensionless) age-specific contact function normalized over the total host population. In order to use this continuous function of age in the modelling process, the human population is partitioned into n age groups each of width 1 year and mid-value a 1 through a n so that n = 60 and the upper end of the of the n-th class is 60 years, with a i denoting the i-th age group,
The function ρ(a i ) represents the relative contact rates distributed over age, and following Chan et al. (1995)  it has equation,
The expression for ρ(a i ) depends on two shape parameters, namely β and c, which together determine its functional form with host age. (Further details can be found in Supplementary Protocol S2 of .) The function Λ(a) denotes the yearly average number of (egg-producing) worms acquired per person of age a and comprises the product of the contact rate with infective stages, the probability of infection upon contact, and the average population of cercariae in the environment; information relating to the number of contacts per unit time is subsumed within λ B . We are not focussing on estimating absolute values of FOI but the relative changes at each point of follow-up. It is assumed that praziquantel instantaneously reduces the adult worm burden of S. mansoni by 95 % in all treated hosts .
At baseline (prior to treatment), ζ 0 = 1, and at follow-up years 1, 2, and 3 (FY1, FY2 and FY3), ζ 1, ζ 2, and ζ 3 indicate the ratio of the FOI relative to that at baseline, respectively, when data for all three follow-up years were available (e.g. Mali, Uganda). When follow-up data were available for one or two consecutive years after baseline (e.g. Zambia), the changes in the FOI were estimated as described for these years. For Tanzania, treatment was distributed at baseline and at FY1, but not at FY2 or FY3; however, evaluation was conducted at FY3, so an average change in the FOI for FY2 and FY3 is reported here. A value of ζ P lower or greater than 1 indicates, respectively, a reduction or an increase in the FOI from baseline, and a statistically significant reduction or increase is indicated when the entire range of the confidence interval lies, respectively, belowor above 1. Confidence intervals for these parameters were estimated as outlined below.
To understand how the overall changes in the FOI at coarse-grain, macroepidemiological levels compared with those taking place at a fine-grain scale (which are more likely to correspond to individual transmission zones), changes in the FOI were also estimated separately for each of 32 Ugandan sentinel sites with longitudinal data and are reported where the sample size > 20 individuals (Fig. 2).
This dataset was also used to examine whether any programmatic variables were statistically associated with the FOI. A multivariate linear regression model incorporating random effects was used with baseline intensity of infection, treatment coverage and cohort retention rate at each time point as the covariates, and the absolute FOI at each sentinel site and time point as the dependent variable. Therapeutic treatment coverage (the proportion of people treated) was available only at the district level (not at the sentinel site level) and was calculated by dividing the number of people treated (taken from treatment registers) by the targeted number in each district (derived from census figures with a correction for population growth rates) (unpublished data). The random effects of district were included in order to control for any geographical clustering at this level . Regression analyses were carried out at each of the three follow-up time points, and were weighted by the number of individuals at each sentinel site. The most parsimonious yet adequate model was chosen by comparing Akaike Information Criterion (AIC) scores . P-values were considered significant at the 0.05 level.
The model described in Eqs. 1, 2, 3 and 4 above was modified so the number of eggs (per 10 ml urine) produced per adult female worm was set to 3.60 , and the efficacy of praziquantel to 99 % . This assumed efficacy was shown to provide a better fit to the data (see next subsection for a description of fitting approach) for the most complete S. haematobium dataset (that of Burkina Faso; log-likelihood ratio test statistic = 12.54, p-value of χ 2 < 0.001) and was retained for all S. haematobium areas. This assumption is conservative with regards to estimates of reduction in the FOI following chemotherapy, i.e. the larger the assumed proportion of parasites killed with treatment, the greater the reinfection rate would need to be for parasite load to return to observed levels. Schistosoma haematobium-parameterised models were fitted to data from Burkina Faso and Tanzania (three consecutive years of follow-up), Mali and Niger (two years), and Zambia (one year).
The cross-sectional cohorts at baseline consisted of both children and adults in order to provide the profile of age-related infection intensity for each schistosome species, endemicity area, and country. The age-stratified model of Eqs. 1, 2, 3 and 4 was fitted simultaneously to the longitudinal cohorts and the cross-sectional baseline data using maximum likelihood estimation of the parameters of interest. The model was fitted to individual host data (taking into account the high degree of parasite overdispersion observed and estimated in each dataset (see below and SI)) to estimate the following: the baseline FOI, λ B , the two shape parameters of the contact function (ββ and c– Eq. 4), and the change in the annual FOI after each round of chemotherapy (ζ 1, ζ 2, and ζ 3) relative to that at baseline. Uncertainty around the parameters was estimated using the Fisher Information Matrix .
The multi-dimensional parameter space was explored using the Latin Hypercube sampling method [44, 45]. As part of this fitting approach, the infection intensity observations of individuals were compared to the model-derived, age-specific mean intensity of infection. Ninety five percent confidence intervals around each of the parameters were calculated using the Fisher Information Matrix . Confidence intervals around the model output (mean egg output) were estimated by re-running the model and randomly selecting parameters from within their 95 % CI bounds. Runs which generated likelihood values not statistically significantly different from the best fit run (tested using a Χ 2 distribution with the appropriate degrees of freedom) were used to construct 95 % CI around the model outputs . The maximum and minimum mean egg output at each timepoint from these runs constituted the upper and lower confidence intervals respectively.
The FOI is a dynamic entity, decreasing immediately after treatment and increasing in inter-treatment periods. Therefore, the estimated values correspond to an average across the relevant periods (each period being one year for annual treatment unless otherwise stated) and were corrected for the ageing of the cohort. Given that these are longitudinal cohorts (with no replacement of the youngest ages), the average age of participants will inevitably increase, and schistosomiasis infection intensity is known to be strongly dependent on host age  with an increase in exposure typically experienced by children between the ages of 5 – 15 years. Therefore, the fitted mathematical model takes this into account by allowing the age of the cohort to increase over time. Not correcting for age can lead to underestimates of the FOI reductions (or overestimates if the FOI increases) .
Model-derived changes in the average infection intensity were calculated to help understand the impact of treatment. Of particular importance are the estimated changes in the proportion of individuals harbouring high infection intensity (defined as the prevalence of hosts excreting ≥400 epg for schistosomiasis mansoni and ≥50 e/10 ml for schistosomiasis haematobium), as those individuals are deemed to be the ones more likely to develop and suffer morbidity , though even light infections can be associated with significant morbidity .
Models were parameterized using parasite overdispersion values calculated from prevalence-intensity relationships for each endemicity area, parasite species, and country (data not shown). Overdispersion of schistosome distribution among hosts was assumed to be reasonably approximated by the negative binomial distribution, with the value of the overdispersion parameter (denoted by k, an inverse measure of the strength of overdispersion), allowed to vary with the mean intensity of infection following either a linear (k = k 0 + k 1 m), or a power function (k = k 0 + k 1 m k2), where m denotes the arithmetic mean intensity of infection , measured in epg or e/10 ml.
Data used for the analysis in this paper were collected as part of the routine monitoring and evaluation activities of the countries’ schistosomiasis control programmes. Ethical approval for this was provided by Imperial College Research Ethics Committee (ICREC_8_2_2, EC No. 03.36, R&D No. 03/SB/003E) and by the ethical review boards of the Ministries of Health of the respective endemic countries.
A comparison between the baseline values of infection intensity and prevalence of heavy infection in those individuals in the longitudinal cohort and those lost to follow-up was conducted using the most extensive datasets, namely the cohorts for S. mansoni in Uganda and for S. haematobium in Burkina Faso (Fig. 3). In Uganda (Fig. 3a and b) the intensity of infection was 29 % higher in those children who were lost to follow-up (301 epg [95 % CIs: 271, 330 egg] vs. 233 epg [95 % CIs: 205, 261], Z = 3.27, p-value = 0.001), driven primarily by a significant difference in the egg counts of high intensity infections (27.2 % higher; 843 epg [95 % CIs: 755, 930 egg] vs.663 epg [95 % CIs: 574, 752 egg], Z = 2.82, p-value = 0.005). However, there were no significant differences in the prevalence of heavy infection between the two groups (10.1 % higher in those lost to follow-up; 18.2 % [95 % CIs: 16.7 %, 19.8 %] vs.16.6 % [95 % CIs: 14.8 %, 18.3 %], Z = 1.41, p-value = 0.16).
In Burkina Faso, no significant differences were observed in infection intensity between those in the longitudinal cohort, and those who were lost to follow-up. With regard to the prevalence of heavy infection, there was a non-significant higher prevalence in those lost to follow-up in high intensity areas (33.9 % vs. 29.8 %, Z = 1.61, p-value = 0.10), and a statistically significantly lower prevalence in low intensity areas (1.09 % vs. 4.08 %, Z = 2.21, p-value = 0.02).
There was no significant difference in the cohort retention rate with respect to age in Uganda (logistic regression, coefficient = −0.0085, Z = −0.506, p-value = 0.61). However, in Burkina Faso the retention rate was statistically significantly and negatively associated with age (coefficient = −0.1165, Z = −2.888, p-value = 0.004).
Changes in relative FOI with treatment
Reductions in the FOI of S. mansoni in Uganda at a macroepidemiological level have been estimated previously . In summary, large and statistically significant reductions were observed across all three infection intensity areas –following one round in low intensity areas (76 % reduction), and following two rounds in moderate (66 % reduction) and high (63 % reduction) intensity areas (Table 2). A similar situation was observed in Mali, with substantial and significant reductions following two rounds of treatment in low (82 % reduction) and high/moderate intensity areas (40 %). This contrasts with Tanzania where large increases in the FOI were observed following one round of treatment (although a second round resulted in sizeable reductions in low intensity areas). Modest increases in the FOI were observed in Zambia, both in the moderate and low intensity areas.
In Burkina Faso, very extensive reductions in the FOI were observed for S. haematobium, even following just a single round of treatment (Table 3), with infection and transmission levels dropping to virtually zero. Here, there was no treatment at FY1 so the values of ζ1 (1.3 and 0.5 %) and ζ 2 (12.4 and 25.3 %) represent the relative ratio of the FOI one and two years after a single treatment round. Even after a two-year treatment gap, reinfection levels were markedly suppressed (close to 100 % reduction in high and low intensity areas in the first year; 88 and 82 % respectively in the second year). In Mali there were marked and statistically significant reductions following one round of treatment in high intensity areas, and following two rounds in low intensity areas. In Niger reductions were modest (and not statistically significant) in high intensity areas, and considerable and statistically significant in low intensity areas. In Tanzania there was a 50 % reduction following one and two rounds of treatment, which increased to 65 % following three rounds. Zambia presents a more confusing picture; there was a relatively small reduction in high intensity areas (27 %) following one round of treatment, but a large increase in the FOI in low intensity areas (more than twice the baseline value).
Heterogeneity in changes in the FOI at a finer geographical scale
Changes in the FOI for each of the 32 sentinel sites from Uganda are displayed in Table 4. Heterogeneity was observed particularly in high intensity areas, where the FOI following one treatment round—as a proportion of that at baseline—ranged from 0.179 (i.e. an 82.1 % reduction) to 1.993 (i.e. a nearly twice as large a value as that at baseline). This variation may be due to true heterogeneity between the sites or the impact of small statistical size in some areas.
Significant increases in the FOI were observed in some sentinel sites/schools following treatment, although as may be expected the number of such sites diminished with successive treatment rounds. In Table 4, changes in the FOI are reported together with changes in the intensity of infection; there were no signficant increases in infection intensity following treatment (although there were some non-signficant increases) and reductions in infection intensity tended to increase in magnitude with successive treatment rounds.
Factors influencing changes in the FOI
Treatment coverage data were collated at the district level in Uganda and the reported figures were generally high each year. At baseline, the average therapeutic coverage in those districts within which the sentinel sites were located was 83 % (ranging from 63 to 93 %), at FY1 it was 90 % (87–97 %), at FY2 82 % (63–99 %, and at FY3 86 % (78–98 %).
Results of multivariate regression analyses examining which programme-relevant factors may influence the FOI are shown in Table 5. No significant associations between district-level treatment coverage and the FOI were observed, or between the cohort follow-up rate (the retention rate of individuals in the cohort) and the FOI. At all three time points there was a statistically significant and positive association between the baseline intensity of infection and the FOI (FY1: estimate = 0.047, t-value = 6.434, p-value < 0.001; FY2: estimate = 0.022, t-value = 4.487, p-value < 0.001; FY3: estimate = 0.022, t-value = 3.187, p-value = 0.004).
For model selection, the model that best described the data was selected for each timepoint separately, using a step function in R to compare AIC values. For the FOI at FY1, the best fitting model included only the baseline infection intensity of the cohort (estimate = 0.047, t-value = 6.66, p-value < 0.001). The same situation was found for the FOI at FY2 (estimate = 0.0218, t-value = 4.70, p-value < 0.001) and at FY3 (estimate = 0.0236, t-value = 3.74, p-value = 0.001).
Changes in intensity of infection
The temporal trends of infection intensity following treatment are shown in Fig. 4. Upon treatment an instantaneous reduction in infection is modelled, followed by reinfection throughout the year prior to the next treatment round. For S. mansoni infections, significant overall reductions were observed in all countries except in Tanzania, where the intensity of infection remained stable throughout the monitored duration of control (for high/moderate intensity areas; Fig. 4a), or stable after one treatment and reduced after two treatment rounds (low intensity areas; Fig. 4b). Similarly, reductions in the infection intensity of S. haematobium were observed in all countries except in those areas of Zambia with a low intensity of infection at baseline, where there was a slight increase following a single round of treatment (Fig. 4d).
Reduction in the prevalence of heavy infection
As a proxy for morbidity, the proportion of individuals harbouring ‘heavy’ parasite burden (itself measured by egg load excreted in stool and urine) fell sharply in most areas (Fig. 5). For S. mansoni there were substantial reductions in Uganda and Mali, and slight increases in Tanzania, where it remained around 30 % in high/moderate intensity areas, and around 0.5 % in low intensity areas. For S. haematobium, the prevalence of heavy infection intensity fell everywhere, including in Zambia (where there had been relative increases in the FOI), from 20.5 to 4.8 % in high/moderate intensity areas and from 2.9 to 2.1 % in low intensity areas.
The impact of preventative chemotherapy with praziquantel on the level of schistosomiasis infection intensity, prevalence and morbidity in SCI-assisted programmes has been published previously (e.g.  and references therein). In this paper, we assessed the impact on transmission according to schistosome species, baseline endemicity level, country (and sentinel site for Uganda) for six SSA countries that have been assisted by the SCI to implement MDA programmes with praziquantel.
Representativeness of the SCI longitudinal cohorts
In areas with higher infection intensity, children lost to follow-up had a significantly higher parasite load than those who remained in the cohort. These differences may have been caused by those heavily infected children being too ill to come back for treatment, being less likely to be enrolled in schools or having a higher degree of absenteeism. In Uganda, the higher infection intensity in those lost to follow-up could also have been caused by the movement of peripatetic fishing communities who historically suffer from high infection intensity and present only occasionally for treatment. Conversely, where infection levels were higher in the longitudinal cohort in areas of low endemicity, infected children may have been more likely to feel unwell and therefore more likely to seek treatment than their uninfected peers.
There was no difference in cohort retention rate with host age in Uganda. However, in Burkina Faso older children were less likely to return for treatment at each follow-up year. As older children typically have heavier infections, infection intensity in follow-up years may have been underestimated, leading to overestimations of the reductions in the FOI, although the differences in follow-up were small and the age-range relatively narrow.
It may be argued that those individuals recruited into the SCI cohorts do not represent the wider community because school-aged children are exposed to more extensive health messages around avoiding risky water contact. One approach to overcome this potential bias is to use putatively untreated 6-year olds recruited into the cohort each year as a proxy for the community. This approach was attempted in an earlier paper  but was not as useful as expected, likely due to the uncertainty about their treatment and infection history.
Variation in relative changes of the FOI at broader- and fine-grain epidemiological levels
Reductions in the FOI relative to baseline values were observed in most, but not all, combinations of country, species, endemicity levels, and treatment rounds. Such reductions will benefit not only those who receive treatment, but also those individuals living in the same area (or accessing the same transmission site) who do not receive treatment, including non-enrolled school children who do not present for treatment, those too young to be included in MDA programmes, or adults living in an area receiving school-based treatment only. These benefits are often missed from the evaluation of schistosomiasis (and other helminthiases) control programmes (though see ). Exceptions to these reductions are reported for Tanzania (particularly for S. mansoni and S. haematobium to a lesser extent), and Zambia (for S. mansoni and S. haematobium in low intensity areas), where relative increases in the FOI were observed (although the average intensity of infection remained stable or dropped). The results from the control programme in Tanzania were affected by very low coverage rates in some areas (of the order of 10–20 %, unpublished data), due to adverse publicity against the aims of the control programme, thought to have been instigated as part of a politically-motivated campaign . A further possible explanation is that of treatment failure in these areas. However, evidence to the contrary is provided by population genetic studies of schistosomes from Tanzanian schools , which demonstrated that parasites obtained from children after treatment were not closely related to those obtained at baseline, suggesting reinfection rather than clearance failure. Increases in the FOI were recorded in Zambia although the average infection intensity was reduced significantly in the cohorts followed up for S. mansoni and only increased marginally significantly in the S. haematobium cohort. In low intensity areas, treatment was targeted at school-aged children only, which will likely lead to a smaller impact on infection intensity/FOI as only a smaller proportion of the circulating pool of parasites would be cleared by praziquantel.
It cannot be discounted that the differences in sampling strategy (e.g. number of Kato Katz slides taken from a number of stool sample) had an impact on the estimates of parasite intensity and therefore on changes in the FOI. This will be the subject of a future analysis.
Considerable heterogeneity in relative changes in the FOI was also observed at the sentinel site/school level in Uganda. Possible explanations include variation in epidemiological, ecological, programmatic (e.g. levels of compliance) and locale-specific factors. In addition, variability will inevitably be introduced by the stochastic nature of the infection process given the smaller size of these cohorts. The extent of the heterogeneity between these sites provides a cautionary note to the interpretation of the results of control programmes at a more macroepidemiological level, i.e. schistosomiasis is fundamentally a focal disease. This is particularly important when examining the impact of control interventions at the level of transmission zones or contemplating the possibility of elimination, as the presence of transmission hot-spots and the possible connectivity between these through movement of definitive and/or intermediate hosts may help maintain and resupply the infection .
Factors influencing changes in the FOI
The lack of clear relationships between the estimated FOI and programmatic factors such as treatment coverage was perhaps surprising, although this was undoubtedly affected by the relatively small range of treatment coverage observed, its aggregation level (district rather than school/community), and its dependence on dated census reports. Further work is required as and when treatment coverage data become available at a more disaggregated level. In other helminth diseases relying on MDA for their control (e.g. onchocerciasis), not only are the levels of therapeutic coverage important, but those of systematic non-compliance are crucial in influencing the long-term trends of infection intensity and prevalence as shown in modelling studies [54, 55]. However, the relationship between the FOI and the baseline endemicity level is interesting and consistent with findings in other helminth infections for which the duration of treatment necessary to eliminate the infection for various initial endemicity levels has been investigated .
Model structure and parasite biology
Worm lifespan is a parameter that plays a key role in driving the outcomes of the model; the shorter the lifespan the higher the corresponding estimates of the FOI, yet there is still significant uncertainty as to the value for this parameter, with 2 to 10 years often quoted as a plausible range for the mean life span [3, 4]. However, some reported cases of infection (in the absence of reinfection) exceed 30 years [57, 58], suggesting that the distribution may be heavily right-skewed. Studies to elucidate the patterns of worm lifespan are required , not just in terms of average age, but also the shape of the distribution of worm ages. This will be important to consider for any future elimination programmes in order to predict the required length of control interventions (e.g. vector control, chemotherapy), as has been the case in other helminth infections such as lymphatic filariasis  and onchocerciasis [61, 62], particularly in view of the fact that schistosomiasis programmes are starting to consider elimination of the infection as a goal [63, 64].
There are likely to be density-dependent processes operating on the worm’s lifecycle, such as mating probability, parasite establishment (via acquired immunity elicited by established worms), and parasite fecundity. A reduction in parasite intensity following treatment will lead to a relaxation of the negative density-dependent processes (an increase in the per-capita establishment or reproductive success of the parasite), and therefore smaller reductions in FOI than might otherwise be expected. The modelling approach utilised here defines the FOI in terms of the number of worms which establish per host per unit time which encapsulates all of the above processes (as has also been utilised in onchocerciasis studies , rather than in terms of the host’s exposure to parasite transmission stages, which is sometimes referred to as the transmission potential [66, 67]. We contend that this definition is more useful for morbidity control programmes as it is the established worms that drive morbidity and onward transmission, rather than the parasites to which hosts are exposed.
We still have an incomplete understanding of how the immunity of human hosts influences the rate of reinfection following treatment. Therefore, changes in FOI need to be interpreted carefully. The reduced rate of reinfection following treatment could be explained either through a decrease in environmental transmission or a decrease in the treated host population’s susceptibility to reinfection. PZQ treatment is known to release somatic parasite antigens which may elicit protective responses that facilitate resistance to reinfection. However, it is far from clear how much this immunological response may influence the rate of Schistosoma establishment, development, or fecundity.
Parasite ‘strains’ and hybridization
There is an increasing body of evidence for the existence of some degree of within-species differentiation in the Schistosoma genus. Differing phenotypes and genotypes of parasites have been reported as occurring in different regions of SSA (for S. mansoni [68, 69], for S. haematobium [69–71] and for S. japonicum ). It is likely that such parasite ‘strains’ may vary in their susceptibility to praziquantel. Epidemiological and ecological conditions, such as water-contact behaviour, the resilience of snail intermediate hosts to environmental perturbations, and the extent and permanence of water bodies will inevitably differ between areas. In addition, secular changes such as rainfall patterns; water and sanitation programmes; human population distribution and migration; patterns of coinfection; and the motivation and experience of the programme staff will all affect the success of a control programme. These all constitute examples of the challenges in translating what are undoubtedly clinically efficacious interventions into effective community-level programmes .
Allayed to this is the issue of hybridization between schistosome parasite species, often between human and non-human schistosome species. There is reported evidence of natural hybridization between S. haematobium and the livestock species S. bovis in Senegal , between S. haematobium and S. guineensisin Cameroon [75, 76], between S. haematobium and S. curassoni of livestock in Senegal , between S. mansoni and the rodent parasite S. rodhaini in Kenya , and of mating interactions between S. mansoni and S. haematobium in hamster models . Additionally, co-infection of S. mansoni and S. haematobium has been found to have an impact on the infectivity and morbidity of single versus mixed infections [80–82].
Control versus elimination
Although annual praziquantel treatment resulted, in the majority of areas, in relative reductions in the FOI, in the absence of on-going treatment and/or measures that more permanently reduce exposure, parasite acquisition and infection intensity will likely return towards baseline values. Recently there has been an increased focus on elimination of schistosomiasis using a combination of intervention strategies, such as has occurred in Morocco [83, 84], Japan , and in large areas of China . How feasible elimination of infection is in the transmission heartland of SSA is less certain. However, there are now programmes that aim to identify the kinds of integrated strategies (such as MDA, water and sanitation improvement, and comprehensive health education messages) that are needed to eliminate schistosomiasis in SSA, particularly in more isolated foci such as on the Unguja island of Zanzibar [63, 64].
The results from the models presented here can help towards this end by identifying areas where MDA alone may be sufficient to push transmission below thresholds of no return (known as transmission breakpoints [10, 11]), or more pragmatically, below operational thresholds for elimination that would indicate the cessation of MDA and the commencement of post-MDA surveillance. This approach has been taken in other helminthiases such as onchocerciasis to identify, through modelling studies, epidemiological scenarios that would require annual or biannual MDA, or the addition of other complementary interventions [55, 87]. We advocate that further modelling work be conducted, including the development of stochastic frameworks, to examine the effect of multiple interventions and of multiple hosts  that could be deployed concurrently or in staggered regimes, and to identify transmission breakpoints in each area, as well as the impact of chance events such as stochastic fade-out.
Significant reductions in the FOI compared to baseline values, as estimated using a schistosomiasis transmission model, were reported in many, but not all, of the SCI cohorts across SSA for both S. mansoni and S. haematobium infections following successive rounds of praziquantel treatment. These reductions will benefit those individuals who receive treatment as well as those who do not, an aspect of MDA that is often missed from its analysis and advocacy, and which is crucial for the quantification of the cost effectiveness of interventions . A wide range of values were observed, likely reflecting the locale-specific ecological, epidemiological, and programmatic conditions, such as the differing approaches to implementation and successes of the various countries’ control programmes. Changes in the FOI at a finer scale also showed great heterogeneity, reflecting the focality of schistosomiasis transmission, and the stochastic nature of the infection process. The application of transmission dynamics models, fitted to longitudinal M&E data, constitutes a helpful tool in the evaluation of large-scale schistosomiasis control and elimination programmes.
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We thank all those children and adults who agreed to take part in the monitoring and evaluation studies as part of the SCI-assisted programmes. We also thank all of the technicians and parasitologists in all countries who expertly collected the data. We thank Dr. Manoj Gambhir for advice on statistical and modelling techniques.
The authors declare they have no competing interests.
JPW, AF and FMF conceived the design of the data collection cohorts, and initiated their implementation. FMF, NBK, MS, AG, ST, UN, JM, LB, and EB-O carried out the collection of data over multiple years from the respective countries. MDF, TC, JW, and M-GB undertook analysis and the interpretation of outputs. MDF, TC, JW, and M-GB drafted the manuscript and revised it critically for content. All authors read and approved the final manuscript.
We thank the Bill and Melinda Gates Foundation (BMGF) for the funding of SCI’s M&E component. MDF also acknowledges PhD funding from the BMGF. MGB thanks the Wellcome Trust (grants 085133/Z/08/Z and 092677/Z/10/Z), and the Royal Society-Leverhulme Trust for a Capacity Building Africa Award. TSC is an Imperial College Junior Research Fellow. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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French, M.D., Churcher, T.S., Webster, J.P. et al. Estimation of changes in the force of infection for intestinal and urogenital schistosomiasis in countries with schistosomiasis control initiative-assisted programmes. Parasites Vectors 8, 558 (2015). https://0-doi-org.brum.beds.ac.uk/10.1186/s13071-015-1138-1
- Mass drug administration
- Schistosoma mansoni
- Schistosoma haematobium
- Force of infection
- sub-Saharan Africa
- Mathematical modelling