Fig. 6

Time- and dosage-dependent inhibitory effects of rSjCa8 on NO production by macrophages stimulated with LPS. Quantification of the NO signal intensity in response to treatment with 20 μg/ml rSjCa8 for 1, 10, 20, or 30 min (a). Fluorescence of the entire area of RAW264.7 cells that was captured in the microscope field of view was quantified by measuring the fluorescence intensity (b). Dynamic changes in NO release in LPS-stimulated macrophages exposed to increasing levels (0–80 μg/ml) of rSjCa8 observed by confocal microscopy were recorded (c). Data represent means ± SD of three experiments; ∗, P < 0.05; ∗∗∗, P < 0.001 compared with the group not treated with rSjCa8; ###, P < 0.001 compared with the group treated with rSjCa8 for 1 min; ▲▲▲, P < 0.001 compared with the group treated with rSjCa8 for 10 min