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Fig. 4 | Parasites & Vectors

Fig. 4

From: SjCa8, a calcium-binding protein from Schistosoma japonicum, inhibits cell migration and suppresses nitric oxide release of RAW264.7 macrophages

Fig. 4

Impaired migration of RAW264.7 cells caused by rSjCa8 in a transwell chamber assay. Filters were stained by Giemsa reagent (a) and the cells that passed through the filter were counted (b). The capacity of RAW264.7 cells to migrate through transwell filters was significantly inhibited by treatment of cells with 1 μg/ml rSjCa8 (P < 0.01) or 20 μg/ml rSjCa8 (P < 0.001), but not rSj13, compared with the PBS group. The means ± SD of three independent experiments are shown; **P < 0.01 and ***P < 0.001, compared with the PBS group; #P < 0.05, compared with the 1 μg/ml SjCa8 group

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