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Table 1 Target gene, primer combinations, amplified conditions used for PCR amplifications

From: Genetic characterization and molecular survey of Babesia bovis, Babesia bigemina and Babesia ovata in cattle, dairy cattle and yaks in China

Species

Target gene

PCR assay

Primer name

Sequence (5′-3′)

Tm (°C)

Product size (bp)

B. bovis

rap-1a

PCRa

B.borap-1aF1/ B.borap-1aR1

ACGCGAATGGTTGCGTTTCAGA

55

570

GGCTCAGCAACATTGGCTTTCAG

nPCRa

B.borap-1aF2/ B.borap-1aR2

GGTGATTACCACTACTTCGTCAC

55

394

CGTACGAGGTCAAGCTACCGAGCAG

PCRb

B.borap-1afull-F/B.borap-1afull-R

ATGAGAATCATTAGCGGCGTTGTCGGTT

64

1698

TCAGAGGTATCCGGCGGTGTCTTCACCG

nPCRb

B.borap-1aseq-F/B.borap-1aseq-R

TGTACGGATGCTTTACGATTGAC

59

1358

AGTTGAGTCGTTAGACTGAGTGGTA

B. bigemina

rap-1c

PCRa

B.birap-1cF1/B.birap-1cR c

AAGCAGCAGCCGTGGTACAAGCGTTGG

58

657

TTACGACGATCGTTTGAAGTACTTC

nPCRa

B.birap-1cF2/B.birap-1cR c

TGGCGAACTCGCAGACCAAGTAG

60

274

PCRb

B.birap-1cF/B.birap-1cR c

ATGATTCACTACGCTTGCCTCA

57

1530

nPCRb

B.birap-1cseq-F/B.birap-1cR c

TTACGCTGCTTACTACAGCTTCA

57

1054

B. ovata

ama-1

PCRa

B.ovama-1 F1/B.ovama-1R1 c

GGCAGGTGCCTGCGTGGCGATCG

62

600

GAGCAACAACAGCGCCGTAGTAATCACG

nPCRa

B.ovama-1 F2/ B.ovama-1R2

TGATATCGATATCGACCTTGATTC

62

310

GAGCTGTCACCATTGTCCTTAACAC

PCRb

B.ovama-1seqF/B.ovama-1R1 c

GATACGAGGCTGTCGGTAGC

62

1234

nPCRb

B.ovama-1seqF/B.ovama-1R2

58

1041

  1. aFor screening analysis
  2. bFor sequencing analysis
  3. cbold data: The sequences of these primeres appeared twice or more times