Species | Target gene | PCR assay | Primer name | Sequence (5′-3′) | Tm (°C) | Product size (bp) |
---|---|---|---|---|---|---|
B. bovis | rap-1a | PCRa | B.borap-1aF1/ B.borap-1aR1 | ACGCGAATGGTTGCGTTTCAGA | 55 | 570 |
GGCTCAGCAACATTGGCTTTCAG | ||||||
nPCRa | B.borap-1aF2/ B.borap-1aR2 | GGTGATTACCACTACTTCGTCAC | 55 | 394 | ||
CGTACGAGGTCAAGCTACCGAGCAG | ||||||
PCRb | B.borap-1afull-F/B.borap-1afull-R | ATGAGAATCATTAGCGGCGTTGTCGGTT | 64 | 1698 | ||
TCAGAGGTATCCGGCGGTGTCTTCACCG | ||||||
nPCRb | B.borap-1aseq-F/B.borap-1aseq-R | TGTACGGATGCTTTACGATTGAC | 59 | 1358 | ||
AGTTGAGTCGTTAGACTGAGTGGTA | ||||||
B. bigemina | rap-1c | PCRa | B.birap-1cF1/B.birap-1cR c | AAGCAGCAGCCGTGGTACAAGCGTTGG | 58 | 657 |
TTACGACGATCGTTTGAAGTACTTC | ||||||
nPCRa | B.birap-1cF2/B.birap-1cR c | TGGCGAACTCGCAGACCAAGTAG | 60 | 274 | ||
PCRb | B.birap-1cF/B.birap-1cR c | ATGATTCACTACGCTTGCCTCA | 57 | 1530 | ||
nPCRb | B.birap-1cseq-F/B.birap-1cR c | TTACGCTGCTTACTACAGCTTCA | 57 | 1054 | ||
B. ovata | ama-1 | PCRa | B.ovama-1Â F1/B.ovama-1R1 c | GGCAGGTGCCTGCGTGGCGATCG | 62 | 600 |
GAGCAACAACAGCGCCGTAGTAATCACG | ||||||
nPCRa | B.ovama-1Â F2/ B.ovama-1R2 | TGATATCGATATCGACCTTGATTC | 62 | 310 | ||
GAGCTGTCACCATTGTCCTTAACAC | ||||||
PCRb | B.ovama-1seqF/B.ovama-1R1 c | GATACGAGGCTGTCGGTAGC | 62 | 1234 | ||
nPCRb | B.ovama-1seqF/B.ovama-1R2 | 58 | 1041 |