Development of a framework for genotyping bovine-derived Cryptosporidium parvum, using a multilocus fragment typing tool

Table 5 Comparison of fragment sizes obtained when bovine Cryptosporidium parvum DNA was processed in two different laboratories

Marker	MRI allele	MRI		UoG
Marker	MRI allele	Size (bp)	Prevalence (n = 118)	Size (bp)^a	Prevalence (n = 211)
MM5	1	262	78 %	260	69 %
	2	235	22 %	233	29 %
	NA	288	0 %	287	0.5 %
MM18	1	288	73 %	290	56 %
	2	294	22 %	296	2 %
	3	318	4 %	NA	0 %
	NA	299	0 %	302	0.5 %
MM19	1	298	53 %	299	38 %
	2	304	4 %	305	4 %
	3	292	33 %	293	55 %
	4	316	2 %	NA	0 %
	5	270	2 %	269	0.5 %
	6	253	3 %	NA	0 %
	7	281	3 %	281	0.5 %
	NA	310	0 %	311	1 %
TP14	1	296	78 %	297	60 %
TP14	2	305	22 %	306	40 %
MS1	1	361	100 %	362	99 %
	NA	327	0 %	326	0.5 %
	NA	384	0 %	386	0.5 %
MS9	1	455	100 %	444	96 %
	NA	443	0 %	432	1.5 %
	NA	461	0 %	450	2 %

DNA was prepared and initially sized at the University of Glasgow (UoG), then amplified and sized at Moredun Research Institute (MRI). In addition the prevalence of each allele is given for the two studies. Only primary peaks were used to assign allele number
^aAllele sizes are the binned Genescan results

ISSN: 1756-3305