Transmission indices and microfilariae prevalence in human population prior to mass drug administration with ivermectin and albendazole in the Gomoa District of Ghana

Table 1 Oligonucleotide primer sequences and PCR reaction conditions for identification of Anopheles species and Wuchereria bancrofti

Primer for species ID	Sequence 5’ ⇒ 3’	PCR product size (bp)	PCR conditions
Anopheles gambiae s.l. species
Universal primer	GTGTGCCCCTTCCTCGATGT	468	93 °C 3’ followed by 35 cycles (93 °C 30”; 50 °C 30”; 72 °C 1’); 93 °C 30”; 50 °C 30”; 72 °C 10’
Anopheles gambiae s.s.	CTGGTTTGGTCGGCACGTTT	390
Anopheles merus/ melax	TGACCAACCCACTCCCTTGA	464
Anopheles arabiensis	AAGTGTCCTTCTCCATCCTA	315
Anopheles quadrianulatus	CAGACCAAGATGGTTAGTAT	153
Anopheles funestus s.l. species
Universal primer	TGTGAACTGCAGGACACAT		30 cycles (94 °C 30”; 40 °C 30”; 72 °C 30”); 72 °C 10’
Anopheles funestus s.s.	GCATCGATGGGTTAATCATG	460
Anopheles vaneedeni	TGTCGACTTGGTAGCCGAAC	555
Anopheles rivulorum	CAAGCCGTTCGACCCTGATT	400
Anopheles parensis	TGCGGTCCCAAGCTAGGTTC	235
Anopheles leesoni	TACACGGGCGCCATGTAGTT	146
Wuchereria bancrofti
NV-1	CGTGATGGCATCAAAGTAGCG	188	94 °C 3’; followed by 35 cycles (94 °C 1’; 55 °C 1’; 72 °C 2’); 94 °C 1’; 55 °C 1’; 72 °C 10”
NV-2	CCCTCACTTACCATAAGACAAC	188

ISSN: 1756-3305