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Fig. 3 | Parasites & Vectors

Fig. 3

From: T. gondii rhoptry protein ROP18 induces apoptosis of neural cells via endoplasmic reticulum stress pathway

Fig. 3

Analyses of inhibitors on the apoptosis levels of N2a cells. a After the N2a cells were pretreated with or without Z-ATAD-FMK (ZAF) for 6 h, they were transfected with ROP18-GFP or control GFP vector for 24 h. Then the apoptosis of the cells were detected by flow cytometry after Annexin V-PE/7-AAD staining. ROP18-GFP or GFP+ ZAF stands for the N2a cells pretreated with ZAF, and then transfected with either ROP18-GFP or control GFP vector. The plots are from a representative measurement and the quantitative data were expressed as mean ± SD on three different assays (n = 3).*P < 0.05, compared with the controls. b After the N2a cells were treated with or without ZAF for 6 h, they were infected with ROP18-RH or RH tachyzoites at an m.o.i of ~3 for 24 h. Then the apoptosis of the cells were detected by flow cytometry after Annexin V-PE/7-AAD staining. ROP18-RH + ZAF or RH + ZAF stands for the N2a cells pretreated with ZAF, and then infected with either ROP18-RH or RH tachyzoites. The plots are from a representative measurement and the quantitative data were expressed as mean ± SD on three different assays (n = 3).*P < 0.05 vs. RH group; **P < 0.01 vs. ROP18-RH group; # P < 0.05 vs. RH group

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