From: Diagnosis of toxoplasmosis and typing of Toxoplasma gondii
Molecular methods | Main purposes | DNA target regions | References |
---|---|---|---|
Conventional PCR | Species detection | B1 gene, 529Â bp repeat element, 18S rDNA gene, SAG1, SAG2, and GRA1 | |
Real-time PCR | Species detection | B1 gene, 529Â bp repeat element, 18S rDNA gene, SAG1 | |
LAMP | Species detection | 529-bp repetitive element, B1, SAG1, SAG2, GRA1, oocyst wall protein genes | |
Microsatellite analysis | Genotyping | TUB2, W35, TgM-A, B18, B17; M33, IV.1, XI.1, M48, M102, N60, N82, AA, N61, and N83 | [156] |
Multilocus sequence typing | Genotyping | BTUB, SAG2,, GRA6, and SAG3 | |
PCR-RFLP | Genotyping | SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1 and Apico | [18] |
RAPD-PCR | Genotyping | Genomic DNA | [177] |
High-resolution melting (HRM) analysis | Genotyping | B1 gene | [182] |