Diagnosis of toxoplasmosis and typing of Toxoplasma gondii

Parasites & Vectors

Table 1 Summary of serological methods for detection of T. gondii infection

Serological methods	Antigens or antibodies used	Antibody/antigen type tested	References
DT	Live tachyzoite	IgG, IgM, IgA	[33]
MAT	Formalin-fixed tachyzoite	IgG	[39]
IFAT	Killed whole tachyzoite	IgG, IgM	[55, 76]
IHA	Tanned red blood cells sensitized with soluble antigens	IgG	[50]
ELISA	Tachyzoite lysate antigen, recombinant antigens, specific antibodies	IgG, IgM, IgA, antigens	[192, 193]
ISAGA	anti-human IgM antibodies	IgM	[79]
LAT	Soluble antigen coated latex particles	IgG, IgM	[194, 195]
PIA	Antigen coated gold nanoparticles	IgG	[90]
WB	Tachyzoite lysate antigen, recombinant antigens	IgG, IgM	[196]
ICT	Antigens or antibodies labeled with colloidal gold	IgG, ESA	[83, 84]
Avidity test	tachyzoite lysate antigen, recombinant antigens	IgG, IgA, IgE	[100]

ISSN: 1756-3305