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Figure 2 | Parasites & Vectors

Figure 2

From: Successful capture of Toxocara canis larva antigens from human serum samples

Figure 2

Determination PoAb optimal concentration to L2TES capture. 96-well polystyrene plate was coated with several concentrations of PoAb, non-specific binding sites were blocked with 1% skimmed milk; added different concentrations; 10 μg/mL of INP-1E4G4C2 MoAb, anti-mouse IgG-HRP conjugate at a 1:8,000 dilution and the reaction was revealed with chromogen/substrate solution. The reaction was stopped with H2SO4 and the absorbance was measured at 490 nm. The results are shown as the arithmetic media ± standard deviation from three triplicate assays.

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