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Table 6 Diagnostic test performance of MT PCR and HRM analysis

From: Comparison of the performance of three PCR assays for the detection and differentiation of Theileria orientalis genotypes

Outcome

Median (95% PI) a

MT PCR

 

Diagnostic sensitivity (%)

98.9 (97.0, 99.8)

Diagnostic specificity (%)

97.8 (94.5, 99.3)

PCR-HRM analysis

 

Diagnostic sensitivity (%)

97.6 (95.6, 99.0)

Diagnostic specificity (%)

96.5 (94.3, 98.3)

Estimated true prevalence

Group 1 (%)

98.2 (95.5, 99.6)

Group 2 (%)

0 (0, 2.14)

Agreement between tests

 

PABAK in group 1

95.2 (90.2, 98.0)

PABAK in group 2

94.0 (87.3, 97.9)

Conditional dependence between tests b

ρ+

0.301 (0.006, 0.715)

ρ-

0.480 (0.106, 0.772)

Model fit

 

pD

12.8

DIC

35.1

  1. The diagnostic sensitivity of the MT PCR was defined based on a cut-off of ≥ 1 DNA copies.
  2. aPI, probability interval.
  3. b ρ+ is the conditional correlation between MT PCR and PCR-HRM outcomes for infected animals, and ρ- is the conditional correlation for uninfected animals.
  4. PABAK = prevalence-adjusted bias-adjusted Cohen’s kappa (ĸ) statistic.
  5. pD = effective dimension (model complexity).
  6. DIC = Deviance information criterion, a generalisation of Akaike’s Information Criterion (AIC).