Comparison of the performance of three PCR assays for the detection and differentiation of Theileria orientalis genotypes

Table 2 Prevalence and intensities of Theileria orientalis genotypes detected using MT PCR in three anaemic categories

Category ( n ) ^a	Apparent prevalence of genotype % (proportion)				Mean intensity of infection (mean no. of DNA copies)
Category ( n ) ^a	*ikeda*	*chitose*	*buffeli*	*type* 5	*ikeda*	*chitose*	*buffeli*	*type* 5
Severe anaemia^b (49)	100 (49/49)	34.7 (17/49)	100 (49/49)	8.2 (4/49)	445,347	68,915	157,443	88
Mild anaemia^c (31)	93.5(29/31)	38.7 (12/31)	96.8 (30/31)	16.1 (5/31)	429,380	167,705	260,844	9,518
No anaemia^d (50)	66 (33/50)	70 (35/50)	92 (46/50)	26 (13/50)	240,359	44,838	134,136	1,558

^a n, number of animals.
^bSevere anaemia was defined if haematocrit value was < 0.15.
^cMild anaemia was defined if haematocrit value was 0.15-0.24.
^dNo anaemia was defined if haematocrit value was > 0.24.
(Haematocrit data were available for only 130 individuals of the tested 154 cattle from North Island).

ISSN: 1756-3305