Table 2 Primers sets for PCR amplification of CVBD agents

Anaplasma spp./Ehrlichia spp.

EHR16SD: 5'-GGT ACC YAC AGA AGA AGTCC-3'

[27]

EHR16SR: 5'-TAG CAC TCA TCG TTT ACAGC-3'

Bartonella spp.

325 s: 5'-CTTCAGATGATGATCCCAAGCCTTCTGGCG-3'

[28]

1100as: 5'-GAACCGACGACCCCCTGCTTGCAAAGCA-3'

Borrelia burgdorferi s.l.

Outer primers:

[29]

132f: 5'-TGGTATGGGAGTTTCTGG-3'

905r: 5'-TCTGTCATTGTAGCATCTTT-3'

Inner primers:

[29]

220f: 5'-CAGACAACAGAGGGAAAT-3'

823r: 5'-TCAAGTCTATTTTGGAAAGCACC-3'

Babesia spp.

PIRO-A: 5'-AAT ACC CAA TCC TGA CACAGG G-3'

[27]

PIRO-B: 5'-TTA AAT ACG AAT GCC CCCAAC-3'

Hepatozoon spp.

HEP-F: 5'-ATA CAT GAG CAA AAT CTC AAC-3'

[30]

HEP-R: 5'-CTT ATT ATT CCA TGC TGC AG-3'

Leishmania infantum

MC1: 5’-GTTAGCCGATGGTGGTCTTG-3’

[31]

MC2: 5’-CACCCATTTTTCCGATTTTG-3’