Parental genetic diversity of brown trout (Salmo trutta m. fario) brood stock affects offspring susceptibility to whirling disease

Table 1 Oligonucleotides used for microsatellite analysis

Microsatellite locus	Oligonucleotide name	Sequence (5′- 3’)	Annealing temperature (°C)	PCR product size (bp)	Reference
Str-15	Str15FAM	TGCAGGCAGACGGATCAGGC	60	220-226	[41]
Str-15	Str15R	AATCCTCTACGTAAGGGATTTGC	60	220-226	[41]
Str-60	Str60FAM	CGGTGTGCTTGTCAGGTTTC	60	94-104	[41]
Str-60	Str60R	GTCAAGTCAGCAAGCCTCAC	60	94-104	[41]
Str-543	Str543NED	ATTCTTCGGCTTTCTCTTGC	55	118-152	[42]
Str-543	Str543R	ATCTGGTCAGTTTCTTTATG	55	118-152	[42]
SsoSL-417	SsoSL417HEX	TTGTTCAGTGTATATGTGTCCCAT	55	160-192	[43]
SsoSL-417	SsoSL417R	GATCTTCACTGCCACCTTATGACC	55	160-192	[43]
SsoSL-438	SsoSL438FAM	GACAACACACAACCAAGGCAC	55	98-108	[43]
SsoSL-438	SsoSL438R	TTATGCTAGGTCTTTATGCATTGT	55	98-108	[43]
Ssa-85	Ssa85NED	AGGTGGGTCCTCCAAGCTAC	60	104-116	[44]
Ssa-85	Ssa85R	ACCCGCTCCTCACTTAATC	60	104-116	[44]
Ssa-197	Ssa197HEX	GGGTTGAGTAGGGAGGCTTG	60	128-158	[44]
Ssa-197	Ssa197R	TGGCAGGGATTTGACATAAC	60	128-158	[44]
OKI-10	OKI10FAM	GGAGTGCTGGACAGATTGG	55	104-220	[45]
OKI-10	OKI10R	CAGCTTTTTACAAATCCTCCT	55	104-220	[45]

Forward primers were 5’end-labeled with fluorescent dyes FAM, NED and HEX, respectively. R: reverse primer.

ISSN: 1756-3305