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Table 4 Relative abundance of nematode miRNA in fluids in comparison to host miRNAs (reads per million)

From: Extracellular Onchocerca-derived small RNAs in host nodules and blood

miRNA

Sequence

O. ochengi

O. volvulus (Ghana)

O. volvulus (Cameroon)

L. sigmodontis

Ooc-miR-71

UGAAAGACAUGGGUAGUGAGAC[G]3

117.6

39.6

7.3

25.1

Ooc-lin-4

UCCCUGAGACCUCUGCUGCGA

1355.8

29.5

5.4

ND (<0.8)2

Ooc-miR-100d 1

AACCCGUAGUUUCGAACAUGUGU

86.2

127.0

ND (<0.3)2

31.4

Ooc-miR-87-3p

GUGAGCAAAGUUUCAGGUGUUC

26.1

34.4

ND (<0.3)2

ND (<0.8)2

Ooc-miR-100a

UACCCGUAGCUCCGAAUAUGUGU

979.6

41.3

ND (<0.3)2

376.0

Ooc-bantam-a 1

UGAGAUCAUUGUGAAAGCUAUU

12863.4

28.7

ND (<0.3)2

35.3

Ooc-miR-86

UAAGUGAAUGCUUUGCCACAGUCU

65.3

ND (<0.8)2

ND (<0.3)2

44.7

Ooc-miR-263/183

AAUGGCACUAGAUGAAUUCACGG

44.4

ND (<0.8)2

ND (<0.3)2

5.5

Ooc-miR-50-5p

UGAUAUGUCUGAUAUUCUUGGGUU

31.3

ND (<0.8)2

ND (<0.3)2

7.9

Ooc-miR-34

UGGCAGUGUGGUUAGCUGGUUGU

70.5

ND (<0.8)2

ND (<0.3)2

6.3

Ooc-miR-5866

UUACCAUGUUGAUCGAUCUCC[A]3

70.5

ND (<0.8)2

ND (<0.3)2

1.60

 

Total host miRNAs

382791

2472450

5924748

1273839

  1. 1Also found in endemic Ghanaian controls.
  2. 2ND = not detected; the limit of detection is shown in (), based on the number of total host miRNAs sequenced and assuming 2 reads are required to identify a parasite sequence.
  3. 3Brackets indicate heterogeneity in the 3′ terminal nucleotide between datasets.