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Table 2 Peak densities and motility in serial passages of B. miyamotoi in MKP-F medium

From: The relapsing fever spirochete Borrelia miyamotoi is cultivable in a modified Kelly-Pettenkofer medium, and is resistant to human complement

Passage

Peak density#

Motility*

 

LB-2001

HT31

LB-2001

HT31

1

12.51

1.91

901

1001

2

15.2 (2.3)2

14.2 (4.2)3

100 (0)2

96.7 (3.3)3

3

21.6 (0.9)2

14.8 (4.7)3

100 (0)2

100 (0)3

4

14.4 (0.6)2

22.3 (5.5)3

100 (0)2

100 (0)3

5

18.1 (4.4)2

15.8 (4.9)3

100 (0)2

100 (0)3

6

11.6 (0.3)2

20.2 (4.2)3

100 (0)2

96.7 (3.3)3

7

15.0 (1.3)2

21.9 (4.5)3

100 (0)2

100 (0)3

8

20.0 (2.5)2

19.6 (6.0)3

100 (0)2

100 (0)3

9

19.1 (9.1)2

24.0 (7.8)3

100 (0)2

100 (0)3

10

16.9 (5.6)2

17.7 (4.5)3

100 (0)2

100 (0)3

  1. B. miyamotoi strains LB-2001 and HT31 were successfully passaged to P10 multiple times. #Mean (±SEM) ×106 spirochetes/ml as determined by dark-field microscopy. *Motility is depicted as the mean percentage of motile spirochetes (±SEM). The number of individual cultures is depicted in superscript.