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Table 1 Primers used for PCR detection of Toxoplasma gondii oocysts in fecal samples

From: High prevalence of Toxoplasma gondii oocyst shedding in stray and pet cats (Felis catus) in Virginia, United States

Primer pair

TOX4/TOX5

TOXF/TOXR

Forward sequence

5’-CGCTGCAGGGAGGAAGACGAAAGTTG-3’

5’-GGAGGACTGGCAACCTGGTGTCG-3’

Reverse sequence

5’-CGCTGCAGACACAGTGCATCTGGATT-3’

5’-TTGTTTCACCCGGACCGTTTAGCAG-3’

Expected product size

529 bp

129 bp

Reference

[13, 30, 31]

[29]

  1. Sequences of primers used in this study to detect Toxoplasma gondii.