Female Anopheles gambiae antennae: increased transcript accumulation of the mosquito-specific odorant-binding-protein OBP2

Table 3 Expression of AGAP003306 (OBP2) relative to expression of S7 in antennae of female and male A. gambiae in two technical replicates.

Target	Technical replicate	Female			Male			Relative Expression
		Cт Mean	Cт SD	ΔCт Mean	Cт Mean	Cт SD	ΔCт Mean	ΔΔCт	RQ
	Experiment 2	33.314	0.335		35.043	0.125
Reference		35.553	0.105		36.507	0.257
S7	Experiment 3	33.565	0.565		32.053	0.188
		36.502	0.309		31.034	0.400
	Experiment 2	29.774	0.168	-3.540	33.873	0.414	-1.170	-2.371	5.172
AGAP		31.725	0.376	-3.828	34.759	0.191	-1.749	-2.079	4.226
003306	Experiment 3	30.845	0.091	-2.721	33.994	0.309	1.941	-4.662	25.309
		33.167	0.384	-3.335	32.711	0.562	1.678	-5.013	32.293

Cт = (cycle threshold) number of cycles required for the fluorescent signal to reach threshold
Cт Mean = Average Cт from triplicate values on same sample in same PCR reaction
Cт SD = Standard Deviation of triplicate Cт
ΔCт Mean = Cт MeanTarget- Cт MeanReference (target=AGAP003306 or AGAP009629; reference=S7)
ΔΔCт = ΔCт Mean_Female - ΔCт Mean_Male
RQ = Relative Quantification = 2^-(ΔΔCт)

ISSN: 1756-3305