Figure 4

Quantitative real-time PCR validation of PCR array data. Based on the PCR array results, 25 genes were chosen for validation in a separate infestation experiment. Gene expression was measured at 48 and 96 hr p.i. during primary infestations and 48 and 72 hr p.i. during secondary exposures. All significant results are shown. In general, gene modulation supported the array study.