Table 2 Protein identification by MALDI-TOF MS
From: Proteomic analysis of an Aedes albopictus cell line infected with Dengue serotypes 1 and 3 viruses
N° spot DIGE | Entree Swissprot_TrEMBL | Identification | Biological process | Cellular component | Molecular function | Sequence similarities | Pathway | Molecular mass (Da) | pI | MASCOT score | Cover sequence |
|---|---|---|---|---|---|---|---|---|---|---|---|
Protein up-regulated in infected cells | |||||||||||
978* | Q17AU4_AEDAE | Putative uncharacterized protein | 42429 | 6.5 | 82 | 25 | |||||
663* | Q16S95_AEDAE | Quinone oxidoreductase | Oxidation reduction | Oxidoreductase activity, zinc ion binding | 50810 | 5.6 | 121 | 31 | |||
838°, 849° | Q17KK5_AEDAE | Enolase | Glycolysis | Cell surface, phosphopyru-vate hydratase complex | Magnesium ion binding, phosphopyruvate hydratase activity | Belongs to the enolase family. | Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 4/5 | 46877 | 6.3 | 202 | 53 |
605°, 606°, 608° | B0WBF6_CULQU | Putative uncharacterized protein | Protein folding | Chaperone, ATP binding, unfolded protein binding | Belongs to the heat shock protein 70 family | 72845 | 5.9 | 188 | 32 | ||
1150°, 1153° | Q177P3_AEDAE | Phosphoglycerate mutase | Glycolysis | 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase activity | 28582 | 6.6 | 131 | 41 | |||
1181°, 1182° | Q17HW3_AEDAE | Triosephosphate isomerase | Glycolysis | Isomerase, triose-phosphate isomerase activity | Belongs to the triosephosphate isomerase family | Carbohydrate biosynthesis, gluconeogenesis, carbohydrate degradation, glycolysis, D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1 | 26705 | 6 | 161 | 44 | |
935°, 938°, 951° | Q178U8_AEDAE | Fructose-bisphosphate aldolase | Glycolysis | Fructose-bisphosphate aldolase activity | Belongs to the class I fructose-bisphosphate aldolase family. | Carbohydrate degradation, glycolysis, D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4 | 39551 | 8 | 172 | 50 | |
716° | Q16PM9_AEDAE | Chaperonin-60kD | Protein refolding | Cytoplasm | ATP binding, unfolded protein binding, chaperone | Belongs to the chaperonin (Hsp60) family | 61155 | 5.5 | 75 | 20 | |
1011°, 1017° | B0WEB5_CULQU | Glyceraldehyde-3-phosphate dehydrogenase | Glycolysis, oxidation reduction | Cytoplasm | NAD or NADH binding, glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity | Belongs to the glyceraldehyde-3-phosphate dehydrogenase family | Carbohydrate degradation, glycolysis, pyruvate from D-glyceraldehyde 3-phosphate: step 1/5 | 35693 | 8.5 | 114 | 40 |
Protein up-regulated in non-infected cells | |||||||||||
1255# | Q1HR19_AEDAE | Calponin | 20936 | 8.3 | 183 | 73 | |||||
528#, 529# | Q0IER9_AEDAE | Procollagen-lysine,2-oxoglutarate 5-dioxygenase | Oxidation reduction | L-ascorbic acid binding, iron ion binding, oxidoreductase activity acting on paired donors with incorporation or reduction of molecular oxygen, oxidoreductase activity acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen | 82324 | 5.7 | 202 | 25 | |||
867# | Q179F9_AEDAE | Ethanolamine-phosphate cytidylyltransferase | Biosynthetic process | Nucleotidyltransferase activity | 42278 | 6.1 | 102 | 23 | |||
442# | Q16ZG5_AEDAE | Aconitase | Metabolic process | 4 iron, 4 sulfur cluster binding | 99252 | 5.8 | 165 | 23 | |||