Gene-knockdown in the honey bee mite Varroa destructor by a non-invasive approach: studies on a glutathione S-transferase

Table 1 Mortality of V. destructor and the degree of gene knockdown under different RNAi experimental conditions.

Treatment	Application Route	Survival at 48 h (%)	Vd GSTMu knockdown (%)^a
dsRNA in 0.9% NaCl	topical droplet	79 (n = 14)	0% (n = 3)
dsRNA in 0.2% Triton-X100	topical droplet	0 (n = 6)	n/d
distilled water	immersion	0 (n = 15)	n/a
0.2% Triton-X100	immersion	0 (n = 15)	n/a
0.9% NaCl	immersion	80 (n = 15)	n/a
dsRNA in 0.9% NaCl	immersion	76 (n = 21)	87 ± 0.7% (3)
dsRNA	injection	50 (n = 62)	96 ± 3.7% (4)

ISSN: 1756-3305