Figure 4

Transcription of the bovipain-2 gene. Bovipain-2 transcripts were detected by total RNA extraction of in vitro cultured B. bovis merozoites (BboS2P strain), followed by incubation with reverse transcriptase and PCR amplification of the complete ORF (RT+). To rule out DNA contamination, an equal amount of RNA was not incubated with reverse transcriptase and then treated as above (RT-). 1 kb: DNA size standard. The size of the obtained amplicon is marked at the left.