Figure 2

Effect of B. bovis infection on the expression of the R. microplus Bm86 gene. At day 5 of feeding, 6 biological replicates of partially engorged female ticks fed on either B. bovis-infected or uninfected cattle were dissected and gene expression was evaluated in individual guts and ovaries by reverse transcriptase quantitative real-time PCR. The transcription level of Bm86 was calculate as relative quantity using the delta Ct comparative method normalized by the total amount of RNA used to generate the cDNA. Different letters (a, b) above the bars indicate significant statistical differences (t test, P < 0.05).