Figure 2

Markers of apoptosis detected in Plasmodium berghei ookinetes grown in vitro. A. A developing ookinete (retort) exhibiting phosphatidylserine translocation to the outer membrane surface, stained with Annexin V (taken from Arambage et al. [29]). B and C. Ookinetes following a JC-1 assay: the mitochondrial membrane potential is intact in B (orange coloured aggregates (m)) and has been lost in C. D and E. Ookinetes following incubation with the caspase substrate fam-VAD-FMK (CaspaTag), D = caspase +ve/PI -ve, E = caspase +ve/PI +ve. F. An ookinete stained positive for DNA fragmentation using the TUNEL assay (provided by L. Politt).