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Figure 3 | Parasites & Vectors

Figure 3

From: Rhipicephalus microplus salivary gland molecules induce differential CD86 expression in murine macrophages

Figure 3

Inhibition of SGE mediated upregulation of CD86 by MEK inhibitor, PD98059. RAW 264.7 cells were left untreated or treated with 50 μM PD98059 for 1 hr. Cells were then stimulated with or without 5 μg/mL SGE for 1 hr followed by 24 hrs of 100 ng/mL LPS or no LPS. RNA was extracted and real-time PCR performed to assay CD86 and TNFα message levels. N = 3 independent experiments. * P < 0.05 ** P < 0.01.

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