Figure 3From: Rhipicephalus microplus salivary gland molecules induce differential CD86 expression in murine macrophagesInhibition of SGE mediated upregulation of CD86 by MEK inhibitor, PD98059. RAW 264.7 cells were left untreated or treated with 50 μM PD98059 for 1 hr. Cells were then stimulated with or without 5 μg/mL SGE for 1 hr followed by 24 hrs of 100 ng/mL LPS or no LPS. RNA was extracted and real-time PCR performed to assay CD86 and TNFα message levels. N = 3 independent experiments. * P < 0.05 ** P < 0.01.Back to article page