Figure 8
From: The use of yellow fluorescent hybrids to indicate mating in Trypanosoma brucei
Analysis of kinetoplast DNA inheritance. A. Maxicircle fragments resulting from Hin fI digestion of PCR amplified COXI gene. * J10 (RP) maxicircle type; â– 1738 (GP) maxicircle type. Inheritance is uniparental except for Fly 1 clone 3, which has maxicircles of both parental types; this result was confirmed in a second reaction. B. PCR products resulting from amplification of genomic DNA using two different sets of minicircle-specific primers. All hybrid progeny clones have the 1738 (GP) minicircle (top) and the J10 (RP) minicircle (bottom) notwithstanding the type of maxicircles inherited from the parents (indicated by the * and â– symbols as above).